Selective suppression of polymerase chain reaction

K. A. Lukyanov, N. G. Gurskaya, E. A. Bogdanova, S. A. Lukyanov

Research output: Contribution to journalReview articlepeer-review

5 Citations (Scopus)

Abstract

The selective suppression of the polymerase chain reaction and methods based upon it (construction of cDNA libraries from low amounts of biological material, subtractive hybridization and differential display of mRNA, fast cloning of full-size cDNA, chromosome walking, cloning in vitro, and others) are reviewed. These methods display a high effectiveness and, taken together, enable intricate DNA analyses to be performed - from the search for nontrivial sequences to the total sequencing of the corresponding genes.

Original languageEnglish
Pages (from-to)141-147
Number of pages7
JournalRussian Journal of Bioorganic Chemistry
Volume25
Issue number3
Publication statusPublished - Mar 1999
Externally publishedYes

Keywords

  • cDNA subtractive hybridization
  • Chromosome walking
  • Differential gene expression
  • Full-size DNA cloning
  • mRNA differential display
  • Polymerase chain reaction suppression

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