Saccharomyces cerevisiae telomerase subunit Est3p binds DNA and RNA and stimulates unwinding of RNA/DNA heteroduplexes

Yu S. Sharanov, M. I. Zvereva, O. A. Dontsova

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Telomerase is a key participant of telomere length maintenance system in majority of eukaryotes. It synthesizes telomere repeats at 3′-end of telomere DNA according to its own RNA template. In addition to the reverse transcriptase subunit Est2p and telomerase RNA TLC1, yeast telomerase contain Est1p, necessary for telomerase attachment to telomere and telomerase activation, and Est3p, a subunit with unknown function. We have isolated Est3p and examined its biochemical properties. Est3p binds both DNA and RNA oligonucleotides containing telomere repeat sequences and stimulates dissociation of RNA/DNA heteroduplexes. The importance of these properties of Est3p for telomerase function is discussed.

Original languageEnglish
Pages (from-to)4683-4690
Number of pages8
JournalFEBS Letters
Volume580
Issue number19
DOIs
Publication statusPublished - 21 Aug 2006
Externally publishedYes

Keywords

  • Est3p
  • Protein subunit of telomerase complex
  • Telomerase
  • Unwinding
  • Yeast

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