Replication protein A modulates the activity of human telomerase in vitro

M. P. Rubtsova, D. A. Skvortsov, I. O. Petruseva, O. I. Lavrik, P. V. Spirin, V. S. Prasolov, F. L. Kisseljov, O. A. Dontsova

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Our aim was to investigate how replication protein A (RPA) in a wide range of concentration can regulate the activity of human telomerase. We used an in vitro system based on human cell extracts with or without RPA. It has been shown that removal of RPA leads to loss of telomerase activity and addition of RPA restores telomerase activity and at the same time promotes telomerase processivity. However, high excess of RPA inhibited telomerase processivity and caused the synthesis of relatively short DNA fragments (about 50-100 nucleotides). We assume that, together with other telomere-binding proteins, RPA may take part in activation of telomere overhang elongation by telomerase at a certain stage of a cell cycle as well as in regulation of telomere length.

Original languageEnglish
Pages (from-to)92-96
Number of pages5
JournalBiochemistry (Moscow)
Volume74
Issue number1
DOIs
Publication statusPublished - Jan 2009
Externally publishedYes

Keywords

  • Regulation
  • RPA
  • Telomerase
  • Telomeres

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