Regulation of methionine/cysteine biosynthesis in Corynebacterium glutamicum and related genomes

G. I. Kovaleva, M. S. Gel'fand

Research output: Contribution to journalArticlepeer-review


Methionine is an essential amino acid and the universal N-terminal amino acid of proteins. The biosynthesis of methionine is extensively studied in various organisms that could be used in biotechnological production of methionine. Transcriptional regulation of the methionine synthesis in the Corynebacterium glutamicum genome is well studied. The McbR protein is a transcriptional regulator of methionine/cysteine biosynthesis genes. The operon structures for members of the McbR regulon also were predicted. We identified candidate regulatory proteins orthologous to McbR in six additional genomes of the order Actinomycetales. The McbR regulon as well as regulons of the orthologous regulators in related genomes were analyzed using the comparative genomics methods. The obtained data demonstrated multiple and diverse positional rearrangements even in the closest genomes despite early observations of the high level of genome stability in corynebacteria. Moreover, the comparison of the operon structures combined with the regulatory signal analysis allowed us to predict some new potential members of the McbR regulons, such as glutamine amidotransferase and methylthioadenosine nuclease.

Original languageEnglish
Pages (from-to)139-150
Number of pages12
JournalMolekulyarnaya Biologiya
Issue number1
Publication statusPublished - Jan 2007
Externally publishedYes


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