Red-Shifted Fluorescent Aminated Derivatives of a Conformationally Locked GFP Chromophore

Mikhail S. Baranov, Kyril M. Solntsev, Nadezhda S. Baleeva, Alexander S. Mishin, Sergey A. Lukyanov, Konstantin A. Lukyanov, Ilia V. Yampolsky

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)


A novel class of fluorescent dyes based on conformationally locked GFP chromophore is reported. These dyes are characterized by red-shifted spectra, high fluorescence quantum yields and pH-independence in physiological pH range. The intra- and intermolecular mechanisms of radiationless deactivation of ABDI-BF2 fluorophore by selective structural locking of various conformational degrees of freedom were studied. A unique combination of solvatochromic and lipophilic properties together with "infinite" photostability (due to a dynamic exchange between free and bound dye) makes some of the novel dyes promising bioinspired tools for labeling cellular membranes, lipid drops and other organelles.

Original languageEnglish
Pages (from-to)13234-13241
Number of pages8
JournalChemistry - A European Journal
Issue number41
Publication statusPublished - 1 Oct 2014
Externally publishedYes


  • fluorescence microscopy
  • fluorescent labeling
  • fluorescent probes
  • GFP chromophore
  • solvatochromism


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