Recombinant bacterial RNA polymerase: Preparation and applications

Konstantin Kuznedelov, Konstantin Severinov

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Availability of DNA-dependent RNA polymerase from various bacteria is a key for setting up specific in vitro transcription systems necessary for understanding species-specific transcription regulation. We describe here two main strategies for recombinant RNA polymerase preparation-through in vitro reconstitution and heterologous co-overproduction in Escherichia coli. Both strategies can be used for preparation of large amounts of RNA polymerases from any bacteria for which sequences of rpo (RNA polymerase) genes are known.

Original languageEnglish
Pages (from-to)44-52
Number of pages9
JournalMethods
Volume47
Issue number1
DOIs
Publication statusPublished - Jan 2009
Externally publishedYes

Keywords

  • Aquifex aeolicus
  • Bacterial
  • Co-overexpression
  • In vitro reconstitution
  • RNA polymerase
  • Transcription

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