Real-time PCR monitoring of signal transduction related genes involved in water stress tolerance mechanism of sunflower

Jane Roche, Tarek Hewezi, Andrée Bouniols, Laurent Gentzbittel

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

The study deals with the quantitative expression pattern of genes involved in signaling transduction pathways in response to water stress in leaves and embryos of a water stress tolerant genotype compared to a non-tolerant genotype using real-time quantitative PCR. The experiment was conducted in the field. The results showed a high quantitative up-regulation of genes belonging to protein kinase, phosphatase and transcription factor pathways (from two to 70 fold) only in leaves of the tolerant genotype compared to the non-tolerant genotype. Moreover, genes related to the protein kinase pathway were down-regulated in leaves of the non-tolerant genotype. On the contrary, in seeds, our study showed that the positive regulation of genes related to the signal transduction pathway observed in leaves of the tolerant genotype is turned off, suggesting different transcriptional control of signaling water stress in reproductive organs compared to vegetative organs.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalPlant Physiology and Biochemistry
Volume47
Issue number2
DOIs
Publication statusPublished - Feb 2009
Externally publishedYes

Keywords

  • Drought tolerant genotype
  • qRT-PCR
  • Signal transduction related genes
  • Sunflower
  • Water stress

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