Quantitative tracking of T cell clones after haematopoietic stem cell transplantation

Ilgar Z. Mamedov, Olga V. Britanova, Dmitriy A. Bolotin, Anna V. Chkalina, Dmitriy B. Staroverov, Ivan V. Zvyagin, Alexey A. Kotlobay, Maria A. Turchaninova, Denis A. Fedorenko, Andrew A. Novik, George V. Sharonov, Sergey Lukyanov, Dmitriy M. Chudakov, Yuri B. Lebedev

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

Autologous haematopoietic stem cell transplantation is highly efficient for the treatment of systemic autoimmune diseases, but its consequences for the immune system remain poorly understood. Here, we describe an optimized RNA-based technology for unbiased amplification of T cell receptor beta-chain libraries and use it to perform the first detailed, quantitative tracking of T cell clones during 10 months after transplantation. We show that multiple clones survive the procedure, contribute to the immune response to activated infections, and form a new skewed and stable T cell receptor repertoire.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalEMBO Molecular Medicine
Volume3
Issue number4
DOIs
Publication statusPublished - Apr 2011
Externally publishedYes

Keywords

  • Ankylosing spondylitis
  • Autoimmunity
  • Haematology
  • Therapeutics
  • Transplantation

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