The coupling of MALDI sources to quadrupole/time-of-flight (QqTOF) instruments that has allowed experiments involving two stages of mass spectrometry, including true MS/MS with collision-induced dissociation (CID) was presented. It was found that an additional fragmentation step could be created through the use of a hot matrix or increased laser fluence. It was also found that 'pseudo-MS3', the combination of non-selective fragmentation followed by MS/MS in a MALDI QqTOF mass spectrometer increased the mass range accessible for peptide sequencing. Several examples from the field proteomics were described including unambiguous identification of a novel phosphorylation site from a protein phosphorylated in vitro.
|Number of pages||2|
|Publication status||Published - 2002|
|Event||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States|
Duration: 2 Jun 2002 → 6 Jun 2002
|Conference||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics|
|Period||2/06/02 → 6/06/02|