Pseudo-MS 3 in a MALDI orthogonal injection quadrupole time-of flight mass spectrometer

Christina S. Raska, Carol E. Parker, R. Marshall Pope, Cai Huang, Jun Han, Gary L. Glish, Christoph H. Borchers

Research output: Contribution to conferencePaperpeer-review

Abstract

The coupling of MALDI sources to quadrupole/time-of-flight (QqTOF) instruments that has allowed experiments involving two stages of mass spectrometry, including true MS/MS with collision-induced dissociation (CID) was presented. It was found that an additional fragmentation step could be created through the use of a hot matrix or increased laser fluence. It was also found that 'pseudo-MS3', the combination of non-selective fragmentation followed by MS/MS in a MALDI QqTOF mass spectrometer increased the mass range accessible for peptide sequencing. Several examples from the field proteomics were described including unambiguous identification of a novel phosphorylation site from a protein phosphorylated in vitro.

Original languageEnglish
Pages563-564
Number of pages2
Publication statusPublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2 Jun 20026 Jun 2002

Conference

ConferenceProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/TerritoryUnited States
CityOrlando, FL
Period2/06/026/06/02

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