Proteomic Profiling of Leishmania donovani Promastigote Subcellular Organelles

Armando Jardim, Darryl B. Hardie, Jan Boitz, Christoph H. Borchers

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

To facilitate a greater understanding of the biological processes in the medically important Leishmania donovani parasite, a combination of differential and density-gradient ultracentrifugation techniques were used to achieve a comprehensive subcellular fractionation of the promastigote stage. An in-depth label-free proteomic LC-MS/MS analysis of the density gradients resulted in the identification of ∼50% of the Leishmania proteome (3883 proteins detected), which included ∼645 integral membrane proteins and 1737 uncharacterized proteins. Clustering and subcellular localization of proteins was based on a subset of training Leishmania proteins with known subcellular localizations that had been determined using biochemical, confocal microscopy, or immunoelectron microscopy approaches. This subcellular map will be a valuable resource that will help dissect the cell biology and metabolic processes associated with specific organelles of Leishmania and related kinetoplastids.

Original languageEnglish
Pages (from-to)1194-1215
Number of pages22
JournalJournal of Proteome Research
Volume17
Issue number3
DOIs
Publication statusPublished - 2 Mar 2018
Externally publishedYes

Keywords

  • correlation profiling
  • glycosome
  • Leishmania
  • mitochondria
  • organelle proteome
  • subcellular fractionation

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