Protein kinase C activity in the lysate of erythrocytes of patients with essential hypertension [EH] and spontaneously hypertensive rats [SHR] was found to be increased by 1.6-2.0 times as compared with normotensive controls. Membrane cytoskeleton alterations observed in the erythrocytes of patients with EH and SHR were revealed in decreased average erythrocyte volume, increase of cup-shaped cell formation, and increase of basal phosphorylation of band 4.9 protein. In addition, the rate of Na+-H+ exchange in erythrocytes of EH patients and SHR was increased by 1.9-fold. In vitro treatment of erythrocytes of healthy donors and Wistar-Kyoto rats [WKY] with protein kinase C activator [12-O-tetradecanoylphorbol-13-acetate] leads to similar changes of cell shape, cell volume, band 4.9 protein phosphorylation and Na+-H+ exchange, as well as to an increase of diS-C3- fluorescence. It may be assumed that alterations of these parameters revealed in primary hypertension are caused by increased activity of protein kinase C.
|Number of pages||10|
|Publication status||Published - 1990|