Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle

G. N. Singina, P. V. Sergiev, A. V. Lopukhov, M. P. Rubtsova, N. P. Taradajnic, N. V. Ravin, E. N. Shedova, T. E. Taradajnic, I. A. Polejaeva, A. V. Dozev, G. Brem, O. A. Dontsova, N. A. Zinovieva

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Abstract: Somatic Cell Nuclear Transfer (SCNT) technique was used to produce the first viable cloned cattle offspring in Russia. Whole-genome SNP genotyping confirmed that the cloned calf was identical to the fibroblast cell line that was used for SCNT. CRISPR/Cas9 approach was subsequently used to knock out genes for beta-lactoglobulin gene (PAEP) and the beta-lactoglobulin-like protein gene (LOC100848610) in the fibroblast cells. Gene editing (GE) efficiency was 4.4% for each of these genes. We successfully obtained single-cell-derived fibroblast colonies containing PAEP and LOC100848610 knockouts, which will be used to produce beta-lactoglobulin-deficient cattle.

Original languageEnglish
Pages (from-to)48-51
Number of pages4
JournalDoklady Biochemistry and Biophysics
Volume496
Issue number1
DOIs
Publication statusPublished - May 2021

Keywords

  • beta-lactoglobulin knock-out
  • Bos taurus
  • gene editing
  • somatic cloning

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