Polyelectrolyte multilayer microspheres as carriers for bienzyme system: Preparation and characterization

Nadezda G. Balabushevich, Gleb B. Sukhorukov, Natalia I. Larionova

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Polyelectrolyte multilayer microspheres were prepared by alternating adsorption of dextran sulfate and protamine on melamine formaldehyde cores followed by the partial decomposition of the core. Peroxidase and glucose oxidase were immobilized in the prepared microspheres, Retention of enzymatic activity of the peroxidase/glucose oxidase system incorporated into the microspheres was demonstrated. Applicability of the bienzyme system immobilized in the microspheres for kinetic glucose assay was shown.

Original languageEnglish
Pages (from-to)1168-1172
Number of pages5
JournalMacromolecular Rapid Communications
Volume26
Issue number14
DOIs
Publication statusPublished - 21 Jul 2005
Externally publishedYes

Keywords

  • Bienzyme system
  • Melamine formaldehyde
  • Microencapsulation
  • Microspheres
  • Polyelectrolytes

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