Polyelectrolyte microsensors as a new tool for metabolites’ detection

L. I. Kazakova, G. B. Sukhorukov, L. I. Shabarchina

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Citation (Scopus)

Abstract

Enzyme based micron sized sensing system with optical readout was fabricated by co-encapsulation of urease and dextran couple with pH sensitive dye SNARF-1 into polyelectrolyte multilayer capsules. Co-precipitation of calcium carbonate, urease and dextran followed up by multilayer film coating and Ca-extracting by EDTA resulted in formation of 3.5-4 micron capsules, what enable the calibrated fluorescence response to urea in concentration range from 10-6 to 10-1 M. Sensitivity to urea in concentration range of 10-5-10-1 M was monitored on capsule assemblies (suspension) and on single capsule measurements. Urea presence can be monitored on single capsule level as illustrated by Confocal fluorescent microscopy.

Original languageEnglish
Title of host publicationPhysics and Chemistry of Classical Materials
Subtitle of host publicationApplied Research and Concepts
PublisherApple Academic Press
Pages219-234
Number of pages16
ISBN (Electronic)9781482257632
ISBN (Print)9781771880459
Publication statusPublished - 1 Jan 2014
Externally publishedYes

Keywords

  • Biological processes
  • Enzymatic reaction
  • Microencapsulated enzyme/dye systems
  • Nanostructured materials
  • Polyelectrolyte multilayer capsules
  • Sensor system

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