Plasma 25-hydroxyvitamin D, hormonal contraceptive use, and the plasma proteome in Caucasian, East Asian, and South Asian young adults

Bibiana García-Bailo, Joseph Jamnik, Laura A. Da Costa, Christoph H. Borchers, Alaa Badawi, Ahmed El-Sohemy

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Vitamin D affects gene expression, but its downstream effects on the proteome are unclear. Hormonal contraceptives (HC), which affect vitamin D metabolism and have widespread effects on the plasma proteome, may confound the association between vitamin D and the proteome. We determined whether HC use modified the association between 25-hydroxyvitamin D (25D) and a panel of 54 high-abundance plasma proteins. Cross-sectional analyses were conducted in healthy, nonsmoking female HC users (n = 216), female HC nonusers (n = 502), and men (n = 301) from Toronto, Canada. Plasma 25D was measured by HPLC-MS/MS, and proteins were measured by LC-multiple-reaction-monitoring (MRM)-MS. The 54 proteins clustered into four distinct proteomic profiles. A positive association was observed between Profile 1, containing positive acute phase proteins, and 25D. In female HC users, a J-shaped association existed between Profile 1 and 25D, but no associations existed in female HC nonusers and men. Twelve proteins were individually associated with 25D in female HC users, but only two were associated with 25D in female HC nonusers and no associations were observed in men. After accounting for hormone dose, only three proteins were associated with 25D. In summary, HC use is an important confounder of the association between circulating 25D and numerous plasma proteins.

Original languageEnglish
Pages (from-to)1797-1807
Number of pages11
JournalJournal of Proteome Research
Volume12
Issue number4
DOIs
Publication statusPublished - 5 Apr 2013
Externally publishedYes

Keywords

  • acute phase proteins
  • hormonal contraceptives
  • plasma proteins
  • proteomics
  • vitamin D
  • young adults

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