Penetration of fullerene C60 derivatives through biological membranes

Igor Andreev, Anna Petrukhina, Alla Garmanova, Alexander Babakhin, Sergey Andreev, Valentina Romanova, Pavel Troshin, Olesya Troshina, Lawrence DuBuske

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Penetration of fullerene C60 in hydrated molecular-colloidal form (FMC) and various C60 water-soluble derivatives (FDs) through membranes of human erythrocytes, platelets and symbiosomes (subcellular organelles of plant origin) were tested. The FDs bearing amino acids induced pronounced depolarization of symbiosome membranes energized with Mg-ATP. In erythrocytes and platelets incubated in K+-free medium in the presence of FCCP, FDs with malonic acid pendants promoted acidification of the intracellular medium thereby simulating an effect of the K+ ionophore valinomycin. Dissipation of pH artificially induced on the plasma membrane of these cells was observed in the presence of C60 - aminobutiric acid which, in addition, strongly stimulated Mg-ATP-dependent generation of membrane potential on symbiosome membranes. C60-Arg was shown to dissipate K+-diffusion potential on erythrocyte membranes induced by valinomycin. Fullerene C60 used in hydrated molecular-colloidal form (FMC) also entered symbiosomes and platelets as evidenced by the quenching of the fluorescence of the Ca2+ indicator chlorotetracycline localized in the interior of these cells. These findings provide evidence for ease of permeation of these fullerene-based compounds through biological membranes from different type cells.

Original languageEnglish
Pages (from-to)89-102
Number of pages14
JournalFullerenes Nanotubes and Carbon Nanostructures
Volume16
Issue number2
DOIs
Publication statusPublished - Mar 2008
Externally publishedYes

Keywords

  • C amino acid derivatives
  • Fluorescent probe
  • Fullerene C
  • Membrane potential

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