One SmpB molecule accompanies tmRNA during its passage through the ribosomes

Elizaveta Y. Bugaeva, Olga V. Shpanchenko, Brice Felden, Leif A. Isaksson, Olga A. Dontsova

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


tmRNA and SmpB are the main participants of trans-translation, a process which rescues the ribosome blocked during translation of non-stop mRNA. While a one-to-one stoichiometry of tmRNA to the ribosome is generally accepted, the number of SmpB molecules in the complex is still under question. We have isolated tmRNA-ribosome complexes blocked at different steps of the tmRNA path through the ribosome and analyzed the stoichiometry of the complexes. Ribosome, tmRNA and SmpB were found in equimolar amount in the tmRNA-ribosome complexes stopped at the position of the 2nd, 4th, 5th or the 11th codons of the coding part of the tmRNA.

Original languageEnglish
Pages (from-to)1532-1536
Number of pages5
JournalFEBS Letters
Issue number10
Publication statusPublished - 30 Apr 2008
Externally publishedYes


  • RNA-protein interaction
  • SmpB
  • tmRNA
  • Trans-translation
  • Translation


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