On subset seeds for protein alignment

Mikhail Roytberg, Anna Gambin, Laurent Noé, Slawomir Lasota, Eugenia Furletova, Ewa Szczurek, Gregory Kucherov

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Abstract-We apply the concept of subset seeds proposed in [1] to similarity search in protein sequences. The main question studied is the design of efficient seed alphabets to construct seeds with optimal sensitivity/selectivity trade-offs. We propose several different design methods and use them to construct several alphabets. We then perform a comparative analysis of seeds built over those alphabets and compare them with the standard BLASTP seeding method [2], [3], as well as with the family of vector seeds proposed in [4]. While the formalism of subset seeds is less expressive (but less costly to implement) than the cumulative principle used in BLASTP and vector seeds, our seeds show a similar or even better performance than BLASTP on Bernoulli models of proteins compatible with the common BLOSUM62 matrix. Finally, we perform a large-scale benchmarking of our seeds against several main databases of protein alignments. Here again, the results show a comparable or better performance of our seeds versus BLASTP.

Original languageEnglish
Article number4752807
Pages (from-to)483-494
Number of pages12
JournalIEEE/ACM Transactions on Computational Biology and Bioinformatics
Volume6
Issue number3
DOIs
Publication statusPublished - Jul 2009
Externally publishedYes

Keywords

  • Local alignment
  • Multiple seeds
  • Protein databases
  • Protein sequences
  • Seed alphabet
  • Seeds
  • Selectivity
  • Sensitivity
  • Similarity search
  • Subset seeds

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