Oligonucleotide primers with G8AE-Clamp modifications for RT-qPCR detection of the low-copy dsRNA

Timofei S. Zatsepin, Anna M. Varizhuk, Vladimir G. Dedkov, German A. Shipulin, Andrey V. Aralov

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    Abstract

    We developed a new technique suitable for improved detection of low-copy dsRNA using modified oligonucleotides as primers in RT-qPCR. Insertion of G8AE-clamp residues into primers significantly improves thermal stability of duplexes with RNA without decrease of hybridization selectivity. The applicability of modified primers is demonstrated for detection of low-copy Kemerovo virus dsRNA.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages281-297
    Number of pages17
    DOIs
    Publication statusPublished - 2019

    Publication series

    NameMethods in Molecular Biology
    Volume1973
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

    Keywords

    • dsRNA
    • G-clamp
    • Modified primer
    • Oligonucleotide
    • RT-qPCR

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