Oligoglutamylation of E. coli ribosomal protein S6 is under growth phase control

Philipp I. Pletnev, Mikhail V. Nesterchuk, Maria P. Rubtsova, Marina V. Serebryakova, Ksenia Dmitrieva, Ilya A. Osterman, Alexey A. Bogdanov, Petr V. Sergiev

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    Ribosomal protein S6 in Escherichia coli is modified by ATP-dependent glutamate ligase RimK. Up to four glutamate residues are added to the C-terminus of S6 protein. In this work we demonstrated that unlike the majority of ribosome modifications in E. coli, oligoglutamylation of S6 protein is regulated and happens only in the stationary phase of bacterial culture. Only S6 protein incorporated into assembled small ribosomal subunits, but not newly made free S6 protein is a substrate for RimK protein. Overexpression of the rimK gene leads to the modification of S6 protein even in the exponential phase of bacterial culture. Thus, it is unlikely that any stationary phase specific factor is needed for the modification. We propose a model that S6 modification is regulated solely via the rate of ribosome biosynthesis at limiting concentration of RimK enzyme.

    Original languageEnglish
    Pages (from-to)61-67
    Number of pages7
    JournalBiochimie
    Volume167
    DOIs
    Publication statusPublished - Dec 2019

    Keywords

    • Bacteria
    • Post-translational modification
    • Ribosome
    • Stationary phase

    Fingerprint

    Dive into the research topics of 'Oligoglutamylation of E. coli ribosomal protein S6 is under growth phase control'. Together they form a unique fingerprint.

    Cite this