Off-axis holographic laser speckle contrast imaging of blood vessels in tissues

Arkady Abdurashitov, Olga Bragina, Olga Sindeeva, Sindeev Sergey, Oxana V. Semyachkina-Glushkovskaya, Valery V. Tuchin

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Laser speckle contrast imaging (LSCI) has become one of the most common tools for functional imaging in tissues. Incomplete theoretical description and sophisticated interpretation of measurement results are completely sidelined by a low-cost and simple hardware, fastness, consistent results, and repeatability. In addition to the relatively low measuring volume with around 700 μm of the probing depth for the visible spectral range of illumination, there is no depth selectivity in conventional LSCI configuration; furthermore, in a case of high NA objective, the actual penetration depth of light in tissues is greater than depth of field (DOF) of an imaging system. Thus, the information about these out-of-focus regions persists in the recorded frames but cannot be retrieved due to intensity-based registration method. We propose a simple modification of LSCI system based on the off-axis holography to introduce after-registration refocusing ability to overcome both depth-selectivity and DOF problems as well as to get the potential possibility of producing a cross-section view of the specimen.

Original languageEnglish
Article number091514
JournalJournal of Biomedical Optics
Volume22
Issue number9
DOIs
Publication statusPublished - 1 Sep 2017
Externally publishedYes

Keywords

  • blood flow
  • cross section
  • depth of field
  • laser speckle contrast
  • numerical refocusing
  • off-axis holography

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