New class of blue animal pigments based on Frizzled and Kringle protein domains

Maria E. Bulina, Konstantin A. Lukyanov, Ilia V. Yampolsky, Dmitry M. Chudakov, Dmitry B. Staroverov, Alexander S. Shcheglov, Nadya G. Gurskaya, Sergey Lukyanov

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The nature of coloration in many marine animals remains poorly investigated. Here we studied the blue pigment of a scyfoid jellyfish Rhizostoma pulmo and determined it to be a soluble extracellular 30-kDa chromoprotein with a complex absorption spectrum peaking at 420, 588, and 624 nm. Furthermore, we cloned the corresponding cDNA and confirmed its identity by immunoblotting and mass spectrometry experiments. The chromoprotein, named rpulFKz1, consists of two domains, a Frizzled cysteine-rich domain and a Kringle domain, inserted, into one another. Generally, Frizzleds are members of a basic Wnt signal transduction pathway investigated intensely with regard to development and cancerogenesis. Kringles are autonomous structural domains found throughout the blood clotting and fibrinolytic proteins. Neither Frizzled and Kringle domains association with any type of coloration nor Kringle intrusion into Frizzled sequence was ever observed. Thus, rpulFKz1 represents a new class of animal pigments, whose chromogenic group remains undetermined. The striking homology between a chromoprotein and members of the signal transduction pathway provides a novel node in the evolution track of growth factor-mediated morphogenesis compounds.

Original languageEnglish
Pages (from-to)43367-43370
Number of pages4
JournalJournal of Biological Chemistry
Volume279
Issue number42
DOIs
Publication statusPublished - 15 Oct 2004
Externally publishedYes

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