Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array

Bernd Zetsche, Matthias Heidenreich, Prarthana Mohanraju, Iana Fedorova, Jeroen Kneppers, Ellen M. Degennaro, Nerges Winblad, Sourav R. Choudhury, Omar O. Abudayyeh, Jonathan S. Gootenberg, Wen Y. Wu, David A. Scott, Konstantin Severinov, John Van Der Oost, Feng Zhang

    Research output: Contribution to journalReview articlepeer-review

    500 Citations (Scopus)


    Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.

    Original languageEnglish
    Pages (from-to)31-34
    Number of pages4
    JournalNature Biotechnology
    Issue number1
    Publication statusPublished - 1 Jan 2017


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