MtQRRS1, an R-locus required for Medicago truncatula quantitative resistance to Ralstonia solanacearum

Cécile Ben, Frédéric Debellé, Hélène Berges, Arnaud Bellec, Marie Françoise Jardinaud, Philippe Anson, Thierry Huguet, Laurent Gentzbittel, Fabienne Vailleau

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


Ralstonia solanacearum is a major soilborne pathogen that attacks > 200 plant species, including major crops. To characterize MtQRRS1, a major quantitative trait locus (QTL) for resistance towards this bacterium in the model legume Medicago truncatula, genetic and functional approaches were combined. QTL analyses together with disease scoring of heterogeneous inbred families were used to define the locus. The candidate region was studied by physical mapping using a bacterial artificial chromosome (BAC) library of the resistant line, and sequencing. In planta bacterial growth measurements, grafting experiments and gene expression analysis were performed to investigate the mechanisms by which this locus confers resistance to R. solanacearum. The MtQRRS1 locus was localized to the same position in two recombinant inbred line populations and was narrowed down to a 64 kb region. Comparison of parental line sequences revealed 15 candidate genes with sequence polymorphisms, but no evidence of differential gene expression upon infection. A role for the hypocotyl in resistance establishment was shown. These data indicate that the quantitative resistance to bacterial wilt conferred by MtQRRS1, which contains a cluster of seven R genes, is shared by different accessions and may act through intralocus interactions to promote resistance.

Original languageEnglish
Pages (from-to)758-772
Number of pages15
JournalNew Phytologist
Issue number3
Publication statusPublished - Aug 2013
Externally publishedYes


  • Bacterial wilt
  • Mapping
  • Medicago truncatula
  • Quantitative resistance
  • Quantitative trait locus (QTL)
  • Ralstonia solanacearum
  • Root pathogen


Dive into the research topics of 'MtQRRS1, an R-locus required for Medicago truncatula quantitative resistance to Ralstonia solanacearum'. Together they form a unique fingerprint.

Cite this