Mouse Trmt2B protein is a dual specific mitochondrial metyltransferase responsible for m5U formation in both tRNA and rRNA

Ivan Laptev, Ekaterina Shvetsova, Sergey Levitskii, Marina Serebryakova, Maria Rubtsova, Alexey Bogdanov, Piotr Kamenski, Petr Sergiev, Olga Dontsova

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    RNA molecules of all species contain modified nucleotides and particularly m5U residues. The vertebrate mitochondrial small subunit rRNA contains m5U nucleotide in a unique site. In this work we found an enzyme, TRMT2B, responsible for the formation of this nucleotide and m5U residues in a number of mitochondrial tRNA species. Inactivation of the Trmt2B gene leads to a reduction of the activity of respiratory chain complexes I, III and IV, containing the subunits synthesized by the mitochondrial protein synthesis apparatus. Comparative sequence analysis of m5U-specific RNA methyltransferases revealed an unusual evolutionary pathway of TRMT2B formation which includes consecutive substrate specificity switches from the large subunit rRNA to tRNA and then to the small subunit rRNA.

    Original languageEnglish
    Pages (from-to)441-450
    Number of pages10
    JournalRNA Biology
    Volume17
    Issue number4
    DOIs
    Publication statusPublished - 2 Apr 2020

    Keywords

    • mitochondria
    • ribosome
    • RNA methyltransferase
    • rRNA
    • tRNA

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