Monoklonal'nye antitela k protektivnomu antigenu B.anthracis sposobny kak neitralizovat', tak i potentsirovat' deistvie sibireiazvennogo letal'nogo toksina in vitro.

Translated title of the contribution: Monoclonal antibodies to B.anthracis protective antigen are capable to neutralize and to enhance the anthrax lethal toxin action in vitro

E. V. Belova, S. A. Dubilei, T. B. Kravchenko, A. V. Kolesnikov, M. I. Zakharova, I. G. Shemiakin

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Anthrax belongs to highly dangerous infections of man and animals. No effective treatment methods for pulmonary types of the disease have been yet developed. The existing anthrax vaccines were designed decades ago and need improvement to fit the large-scale vaccination of population. At the same time, the immunological properties of the anthrax vaccine main component, i.e. of the protective agent, have been poorly studied. We obtained, within the present case study, a panel of mouse monoclonal antibodies to the protective agent and investigated the properties of the highest-affine panel representatives. An unusual phenomenon was detected, which is related with enhancement of the anthrax toxin action on the mouse macrophage-like cell-line in presence of the 1F2 monoclonal antibody. The remaining analyzed antibodies, i.e. 6G8 and 6G7, were found to neutralize effectively the toxin action. The enhancing and neutralizing antibodies were proven to be specific to different domains of the protective antigen and to recognize epitopes in its composition. The antibody-mediated enhancement of the anthrax lethal action is a convincing argument for further development of a new-generation anthrax vaccine. Definition of the linear antigen determinants for neutralizing antibodies in the protective antigens is an important step in the development of the next-generation anthrax vaccine.

Translated title of the contributionMonoclonal antibodies to B.anthracis protective antigen are capable to neutralize and to enhance the anthrax lethal toxin action in vitro
Original languageRussian
Pages (from-to)21-26
Number of pages6
JournalMolekuliarnaia genetika, mikrobiologiia i virusologiia
Issue number3
Publication statusPublished - 2004
Externally publishedYes

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