Modification of human serum albumin under induced oxidation

M. G. Gorobets, L. A. Wasserman, A. D. Vasilyeva, A. V. Bychkova, P. G. Pronkin, A. E. Bugrova, M. I. Indeykina, N. G. Shilkina, M. L. Konstantinova, A. S. Kononikhin, E. N. Nikolaev, M. A. Rosenfeld

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    For the first time, by using the complex of physicochemical methods (mass-spectrometry, differential scanning calorimetry, spectrofluorimetry, method of spectral and fluorescent probes, dynamic light scattering, and UV spectrophotometry), the oxidation-mediated modification of chemical and spatial structure of albumin has been studied. All albumin structural regions are subjected to oxidation, methionine and aromatic amino acids primarily involved in oxidation. The albumin melting shows a decrease in thermal stabilization of the structure and changing of aggregation upon oxidation. The change in physical and chemical properties of albumin under different quantity of the oxidizer has been analyzed.

    Original languageEnglish
    Pages (from-to)231-235
    Number of pages5
    JournalDoklady Biochemistry and Biophysics
    Volume474
    Issue number1
    DOIs
    Publication statusPublished - 1 May 2017

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