Modelling of prebiotic synthesis and selection of peptides under isothermal conditions and thermal cycling mode

O. V. Demina, A. S. Kononikhin, A. V. Laptev, A. A. Khodonov, E. N. Nikolaev, S. D. Varfolomeev

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The model peptide synthesis from mixtures of amino acids was carried out under the thermal cycling and isothermal modes. The compositions of the obtained mixtures of products and the primary amino acid sequence of the synthesized peptides were determined by Fourier transform ion cyclotron resonance mass spectrometry and tandem mass spectrometry in combination with high-performance liquid chromatography with the application of de novo sequencing of the synthesized products. The processes of abiogenous synthesis of peptides were shown to occur under relatively mild temperature conditions and give a substantially less number of peptides as compared with the possible statistical set. The evolution of the system takes place in the process of the synthesis in solid phase with the disappearance of a series of the most unstable peptides. The selection process with the formation of complementary peptides takes place in peptide synthesis under the thermal cyclic mode.

Original languageEnglish
Pages (from-to)422-441
Number of pages20
JournalRussian Chemical Bulletin
Volume61
Issue number2
DOIs
Publication statusPublished - Feb 2012
Externally publishedYes

Keywords

  • amino acids
  • biopolymers
  • mass spectrometry
  • peptide synthesis
  • peptides
  • thermal cycling
  • thermal synthesis

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