Model of the Mediator middle module based on protein cross-linking

Laurent Larivière, Clemens Plaschka, Martin Seizl, Evgeniy V. Petrotchenko, Larissa Wenzeck, Christoph H. Borchers, Patrick Cramer

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


The essential core of the transcription coactivator Mediator consists of two conserved multiprotein modules, the head and middle modules. Whereas the structure of the head module is known, the structure of the middle module is lacking. Here we report a 3D model of a 6-subunit Mediator middle module. The model was obtained by arranging crystal structures and homology models of parts of the module based on lysine-lysine cross-links obtained by mass spectrometric analysis. The model contains a central tetramer formed by the heterodimers Med4/Med9 and Med7/Med21. The Med7/Med21 heterodimer is flanked by subunits Med10 and Med31. The model is highly extended, suggests that the middle module is flexible and contributes to a molecular basis for detailed structure-function studies of RNA polymerase II regulation.

Original languageEnglish
Pages (from-to)9266-9273
Number of pages8
JournalNucleic Acids Research
Issue number20
Publication statusPublished - Nov 2013
Externally publishedYes


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