Mirnas and their gene targets—a clue to differentiate pregnancies with small for gestational age newborns, intrauterine growth restriction, and preeclampsia

Angelika V. Timofeeva, Ivan S. Fedorov, Alexander G. Brzhozovskiy, Anna E. Bugrova, Vitaliy V. Chagovets, Maria V. Volochaeva, Natalia L. Starodubtseva, Vladimir E. Frankevich, Evgeny N. Nikolaev, Roman G. Shmakov, Gennady T. Sukhikh

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Despite the differences in the clinical manifestations of major obstetric syndromes, such as preeclampsia (PE) and intrauterine growth restriction (IUGR), their pathogenesis is based on the dysregulation of proliferation, differentiation, and invasion of cytotrophoblast cells that occur in the developing placenta, decidual endometrium, and myometrial parts of the spiral arteries. To understand the similarities and differences in the molecular mechanisms of PE and IUGR, samples of the placental bed and placental tissue were analyzed using protein mass spectrometry and the deep sequencing of small RNAs, followed by validation of the data obtained by quantitative RT-PCR in real time. A comparison of the transcriptome and proteomic profiles in the samples made it possible to conclude that the main changes in the molecular profile in IUGR occur in the placental bed, in contrast to PE, in which the majority of molecular changes occurs in the placenta. In placental bed samples, significant changes in the ratio of miRNA and its potential target gene expression levels were revealed, which were unique for IUGR (miR-30c-5p/VIM, miR-28-3p/VIM, miR-1-3p/ANXA2, miR-30c-5p/FBN1; miR-15b-5p/MYL6), unique for PE (miR-185-3p/FLNA), common for IUGR and PE (miR-30c-5p/YWHAZ and miR-654-3p/FGA), but all associated with abnormality in the hemostatic and vascular systems as well as with an inflammatory process at the fetal-maternal interface.

Original languageEnglish
Article number729
JournalDiagnostics
Volume11
Issue number4
DOIs
Publication statusPublished - Apr 2021

Keywords

  • Intrauterine growth restriction
  • Mass spectrometry
  • MiRNA deep sequencing
  • MRNA
  • Placenta
  • Placental bed
  • Poly-merase chain reaction in real time
  • Preeclampsia
  • Protein
  • Reverse transcription
  • Small for gestational age

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