Micro-PIV quantification of capillary blood flow redistribution caused by laser-assisted vascular occlusion

Maxim A. Kurochkin, Elena S. Stiukhina, Ivan V. Fedosov, Dmitry E. Postnov, Valery V. Tuchin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Citations (Scopus)

Abstract

We propose μPIV-based technique for quantitative assessment of blood flow redistribution in microcirculatory networks. Our approach is based on per-segment averaging of measured quantities so we can avoid most of problems that are typical for point-wise measurements. The key point of our technique is the digital processing algorithms of recorded data that include: capillary network axial line construction; interrogation regions centering; blood flow velocity local estimate using PIV approach; blood flow velocity calculation by means of averaging over entire vessel segment; the calculation of blood volume flow rate map. We illustrate the application of developed technique with in vivo measurements and blood flow velocity map reconstruction for chorioallantoic membrane (CAM) of chicken embryo, in which the local vascular occlusion was produced using continuous wave laser light irradiation.

Original languageEnglish
Title of host publicationSaratov Fall Meeting 2015 - Third International Symposium on Optics and Biophotonics; and Seventh Finnish-Russian Photonics and Laser Symposium (PALS)
EditorsKirill V. Larin, Alexander B. Pravdin, Valery V. Tuchin, Igor V. Meglinski, Elina A. Genina, Vladimir L. Derbov, Dmitry E. Postnov
PublisherSPIE
ISBN (Electronic)9781510602267
DOIs
Publication statusPublished - 2016
Externally publishedYes
Event3rd International Symposium on Optics and Biophotonics, SFM 2015 and 7th Finnish-Russian Photonics and Laser Symposium, PALS 2015 - Saratov, Russian Federation
Duration: 22 Sep 201525 Sep 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9917
ISSN (Print)1605-7422

Conference

Conference3rd International Symposium on Optics and Biophotonics, SFM 2015 and 7th Finnish-Russian Photonics and Laser Symposium, PALS 2015
Country/TerritoryRussian Federation
CitySaratov
Period22/09/1525/09/15

Keywords

  • Blood flow flux
  • Bright-field microscopy
  • Capillaries blood flow velocity
  • Chorioallantoic membrane assay
  • Digital image processing
  • In-vivo
  • Photothrombotic clot
  • μPIV

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