METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m4C840 12S rRNA residue

Ivan Laptev, Ekaterina Shvetsova, Sergey Levitskii, Marina Serebryakova, Maria Rubtsova, Victor Zgoda, Alexey Bogdanov, Piotr Kamenski, Petr Sergiev, Olga Dontsova

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Mammalian mitochondrial ribosomes contain a set of modified nucleotides, which is distinct from that of the cytosolic ribosomes. Nucleotide m4C840 of the murine mitochondrial 12S rRNA is equivalent to the dimethylated m4Cm1402 residue of Escherichia coli 16S rRNA. Here we demonstrate that mouse METTL15 protein is responsible for the formation of m4C residue of the 12S rRNA. Inactivation of Mettl15 gene in murine cell line perturbs the composition of mitochondrial protein biosynthesis machinery. Identification of METTL15 interaction partners revealed that the likely substrate for this RNA methyltransferase is an assembly intermediate of the mitochondrial small ribosomal subunit containing an assembly factor RBFA.

Original languageEnglish
Pages (from-to)8022-8034
Number of pages13
JournalNucleic Acids Research
Volume48
Issue number14
DOIs
Publication statusPublished - 20 Aug 2020

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