Methodology for urine peptidome analysis based on nano-HPLC coupled to fourier transform ion cyclotron resonance mass spectrometry

Alexey S. Kononikhin, Victoria A. Sergeeva, Anna E. Bugrova, Maria I. Indeykina, Natalia L. Starodubtseva, Vitaliy V. Chagovets, Igor A. Popov, Vladimir E. Frankevich, Patrick Pedrioli, Gennady T. Sukhikh, Eugene N. Nikolaev

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    1 Citation (Scopus)

    Abstract

    Urine is a sample of choice for noninvasive biomarkers search because it is easily available in large amounts and its molecular composition provides information on processes in the organism. The high potential of urine peptidomics has been demonstrated for clinical purpose. Several mass spectrometry based approaches have been successfully applied for urine peptidome analysis and potential biomarkers search. Summarizing literature data and our own experience we developed a protocol for comprehensive urine peptidome analysis. The technology includes several stages and consists of urine sample preparation by size exclusion chromatography and identification of featured peptides by nano-HPLC coupled to Fourier transform ion cyclotron resonance mass spectrometry, semiquantitative and statistical data analysis.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages311-318
    Number of pages8
    DOIs
    Publication statusPublished - 2018

    Publication series

    NameMethods in Molecular Biology
    Volume1719
    ISSN (Print)1064-3745

    Keywords

    • FTICR MS
    • LC-MS/MS
    • Size exclusion chromatography
    • Urine peptidome

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