Metal ion CHElate-aSSisted LIGAtion (CHESS LIGA) for SNP detection on microarrays

Le Thi Hien, Tatiana S. Oretskaya, Timofei S. Zatsepin

Research output: Contribution to journalArticlepeer-review

Abstract

We developed a metal ion chelate-assisted ligation for SNP detection by microarray. An oligonucleotide probe was separated into two 9-10-mers bearing iminodiacetic residues at the gap point. Duplex formation with the DNA target was possible only if nickel ions were added, but a nucleotide substitution opposite the gap point prevented duplex formation. Here we demonstrate the application of this approach for SNP detection (A1298C) within the 5,10-methylenetetrahydrofolate reductase gene on a microarray.

Original languageEnglish
Pages (from-to)4018-4021
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume19
Issue number15
DOIs
Publication statusPublished - 1 Aug 2009
Externally publishedYes

Keywords

  • Chelate
  • Microarray
  • Modified oligonucleotide
  • SNP

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