Maturation of the translation inhibitor microcin

Anastasia Metlitskaya, Teymur Kazakov, Gaston H. Vondenhoff, Maria Novikova, Alexander Shashkov, Timofei Zatsepin, Ekaterina Semenova, Natalia Zaitseva, Vasily Ramensky, Arthur Van Aerschot, Konstantin Severinov

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Microcin C (McC), an inhibitor of the growth of enteric bacteria, consists of a heptapeptide with a modified AMP residue attached to the backbone of the C-terminal aspartate through an N-acyl phosphamidate bond. Here we identify maturation intermediates produced by cells lacking individual mcc McC biosynthesis genes. We show that the products of the mccD and mccE genes are required for attachment of a 3-aminopropyl group to the phosphate of McC and that this group increases the potency of inhibition of the McC target, aspartyl- tRNA synthetase.

Original languageEnglish
Pages (from-to)2380-2387
Number of pages8
JournalJournal of Bacteriology
Volume191
Issue number7
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

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