Mass spectrometry in high-throughput clinical biomarker assays: Multiple reaction monitoring

Carol E. Parker, Dominik Domanski, Andrew J. Percy, Andrew G. Chambers, Alexander G. Camenzind, Derek S. Smith, Christoph H. Borchers

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

21 Citations (Scopus)

Abstract

Clinical biomarker discovery, verification, and validation are facilitated by the latest technological advances in mass spectrometry. It is now possible to analyze simultaneously group of tens or hundreds of biomarkers in a blood sample using multiple reaction monitoring (MRM), a tandem mass spectrometric method. However, these newly-developed methods face new challenges, including standardization, calibration, and the determination of analytical and biological variation. Here we illustrate the background, pre-analytical sample preparation, and biomarker assay development using an MRM-mass spectrometric method. In addition, special attention is given to future standardization methods to enable widespread use of the technology.

Original languageEnglish
Title of host publicationChemical Diagnostics
Subtitle of host publicationFrom Bench to Bedside
EditorsNelson Tang, Terence Poon, Nelson Tang
Pages117-138
Number of pages22
DOIs
Publication statusPublished - 2014
Externally publishedYes

Publication series

NameTopics in Current Chemistry
Volume336
ISSN (Print)0340-1022

Keywords

  • Clinical proteomics
  • Multiple reaction monitoring (MRM)
  • Multiplex
  • Proteomics
  • Quantitation
  • Selected reaction monitoring (SRM)

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