Mass spectrometry based proteome profiling of the exhaled breath condensate for lung cancer biomarkers search

Natalia Zakharova, Anna Kozyr, Anna M. Ryabokon, Maria Indeykina, Polina Strelnikova, Anna Bugrova, Eugene N. Nikolaev, Alexey S. Kononikhin

Research output: Contribution to journalReview articlepeer-review

1 Citation (Scopus)


Introduction: Lung cancer remains the most prevalent cause of cancer mortality worldwide mainly due to insufficient availability of early screening methods for wide-scale application. Exhaled breath condensate (EBC) is currently considered as one of the promising targets for early screening and is particularly attractive due to its absolutely noninvasive collection and possibility for long-term frozen storage. EBC proteome analysis can provide valuable information about the (patho)physiological changes in the respiratory system and may help to identify in time a high risk of lung cancer. Mass spectrometry (MS) profiling of EBC proteome seems to have no alternative in obtaining the most extensive data and characteristic marker panels for screening. Areas covered: This special report summarizes the data of several proteomic studies of EBC in normal and lung cancer (from 2012 to 2021, PubMed), focuses on the possible reasons for the significant discrepancy in the results, and discusses some aspects for special attention in further studies. Expert opinion: The significant discrepancy in the results of various studies primarily highlights the need to create standardized protocols for the collection and preparation of EBC for proteomic analysis. The application of quantitative and targeted LC-MS/MS based approaches seems to be the most promising in further EBC proteomic studies.

Original languageEnglish
Pages (from-to)637-642
Number of pages6
JournalExpert Review of Proteomics
Issue number8
Publication statusPublished - 2021


  • biomarkers
  • Exhaled breath condensate
  • lung cancer
  • mass spectrometry
  • proteomics


Dive into the research topics of 'Mass spectrometry based proteome profiling of the exhaled breath condensate for lung cancer biomarkers search'. Together they form a unique fingerprint.

Cite this