Mass spectrometric approaches combined with photoaffinity labeling and molecular modeling for structural characterization of drug binding sites in HIV-integrase

Christoph H. Borchers, Elisabeth O. Hochleitner, Kenneth B. Tomer, Nouri Neamati

Research output: Contribution to conferencePaperpeer-review

Abstract

The structural characterization of drug binding sites in HIV integrase was discussed. The study was carried out by using the mass spctrometric approaches combined with photoaffinity labeling and molecular modeling. The accurate measurement of the molecular weight of the complex also provides information on the number of molecules inhibitor covalently bound to each molecule of integrase, providing information on the stoichiometry of the complex. It was observed that the MW shift corresponds to a single R61 per molecule of protein, demonstrating that the stocichometry is 1:1, which indicates that there is a single binding site.

Original languageEnglish
Pages351-352
Number of pages2
Publication statusPublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2 Jun 20026 Jun 2002

Conference

ConferenceProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/TerritoryUnited States
CityOrlando, FL
Period2/06/026/06/02

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