Major role of positive selection in the evolution of conservative segments of Drosophila proteins

Georgii A. Bazykin, Alexey S. Kondrashov

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Slow evolution of conservative segments of coding and non-coding DNA is caused by the action of negative selection, which removes new mutations. However, the mode of selection that affects the few substitutions that do occur within such segments remains unclear. Here, we show that the fraction of allele replacements that were driven by positive selection, and the strength of this selection, is the highest within the conservative segments of Drosophila protein-coding genes. The McDonald - Kreitman test, applied to the data on variation in Drosophila melanogaster and in Drosophila simulans, indicates that within the most conservative protein segments, approximately 72 per cent (approx. 80%) of allele replacements were driven by positive selection, as opposed to only approximately 44 per cent (approx. 53%) at rapidly evolving segments. Data on multiple non-synonymous substitutions at a codon lead to the same conclusion and additionally indicate that positive selection driving allele replacements at conservative sites is the strongest, as it accelerates evolution by a factor of approximately 40, as opposed to a factor of approximately 5 at rapidly evolving sites. Thus, random drift plays only a minor role in the evolution of conservative DNA segments, and those relatively rare allele replacements that occur within such segments are mostly driven by substantial positive selection.

Original languageEnglish
Pages (from-to)3409-3417
Number of pages9
JournalProceedings of the Royal Society B: Biological Sciences
Volume279
Issue number1742
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • Double substitutions
  • McDonald - Kreitman test
  • Negative selection
  • Positive selection

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