Magnetic bio/nanoreactor with multilayer shells of glucose oxidase and inorganic nanoparticles

Ming Fang, Patrick S. Grant, Michael J. McShane, Gleb B. Sukhorukov, Vladimir O. Golub, Yuri M. Lvov

Research output: Contribution to journalArticlepeer-review

128 Citations (Scopus)


Organized multilayers of nanoparticles (9-, 20-, and 45-nm-diameter silica 12-nm magnetite) and or glucose oxidase (GOx) were assembled in alternation with oppositely charged polyelectrolytes on 420-nm latex particles. Stepwise growth of the multilayer films on latex was confirmed by microelectrophoresis and transmission electron microscopy. The inclusion of silica layers on latex yields a higher surface area, resulting in greater GOx adsorption and thereby increasing the catalytic activity of the bioreactor. The bioactivity was proportional to the core surface area and also to the number of GOx layers in the shells. Also, the presence of magnetic nanoparticles allows self-stirring of the nanoreactors with a rotating magnetic field and enhances its productivity. The ensemble of GOx and fluorescent dyes in the shells demonstrated the correlation between Ru-bpy fluorescence and glucose concentration in solution.

Original languageEnglish
Pages (from-to)6338-6344
Number of pages7
Issue number16
Publication statusPublished - 6 Aug 2002
Externally publishedYes


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