Long noncoding RNA LL35/falcor regulates expression of transcription factor foxa2 in hepatocytesin normal and fibrotic mouse liver

O. V. Sergeeva, S. A. Korinfskaya, I. I. Kurochkin, T. S. Zatsepin

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    Long noncoding RNAs (lncRNA) play important roles in the regulation of transcription, splicing, translation, and other processes in the cell. Human and mouse lncRNA (DEANR1 and LL35/Falcor, respectively) located in the genomic environment in close proximity to the Foxa2 transcription factor were discovered earlier. In this work, tissue-specific expression of LL35/Falcor lncRNA has been shown in mouse liver and lungs. The use of antisense oligonucleotides allowed us to achieve LL35/Falcor lncRNA downregulation by 90%. As a result, the level of Foxa2 mRNA and protein dropped, which confirms the involvement of LL35/Falcor lncRNA in the regulation of transcription factor Foxa2. We have shown a decrease in the expression of LL35 lncRNA in liver fibrosis, which correlates with the previously published data for mRNA Foxa2. Thus, lncRNA LL35 regulates Foxa2 expression in the liver not only in normalconditions, but also during development of fibrosis, which allows one to consider lncRNA a biomarker of this pathological process.

    Original languageEnglish
    Pages (from-to)66-74
    Number of pages9
    JournalActa Naturae
    Volume11
    Issue number3
    DOIs
    Publication statusPublished - 2019

    Keywords

    • ASO - antisense oligonucleotides
    • Liver
    • Lncrna - long non-coding RNA
    • Non-coding RNA
    • Regulation
    • Transcription factor Foxa2

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