Lipid composition of muscle and fat tissues of duroc pigs (Sus scrofa domesticus Erxleben, 1777) - features and correlations

A. A. Pavlova, A. Vanyushkina, E. A. Ushina, A. N. Egorova, D. Petrova, N. Anikanov, P. V. Mazin

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A key direction in animal genetics and animal breeding is currently the study of metabolic characteristics of animals in combination with their genotyping, which leads to the development of the new specific markers for prediction the individual phenotypic characteristics of animals based on correlation studies. This would further make possible to create new methods for animal phenotyping using lipid analysis techniques to assess the complex effect of environmental and genetic factors (D.P. Lo Fiego et al., 2002; D.P. Lo Fiego et al., 2005; R. Rossi et al., 2002). Data obtained as a result of the analysis of the genetic features of the animal and its metabolic characteristics make it possible to create predictive models for accurate phenotyping of animals and their offspring. In this work we have for the first time performed the comparative non-targeted mass-spectrometry study of the lipid composition of muscle and adipose tissue in Russian Duroc pigs using positive ion registration mode. The aim of the work was to carry out lipidomic analysis of the pig muscle and adipose tissue as an input for predictive models for animal phenotyping. The study was carried out on the samples of adipose and muscle tissue collected post-mortem from 150-180 day-old Duroc boars (n = 9). Samples were taken from three regions of the longest back muscle, three regions of the biceps femoris, and two regions of subcutaneous dorsal fat (72 samples in total). Analysis of the lipid compounds was performed by liquid chromatography coupled with high-precision time-of-flight mass spectrometry, preceded by the methyl tert-butyl ether and methanol extraction of lipids. Type of ionization used was electrospray. A total of 844 mass spectrometry peaks satisfied the quality criteria and were used for the statistical analysis. Peaks were annotated using the LIPID MAPS database search (http://www.lipidmaps.org), with an accuracy of 10 ppm. Statistical analysis shows significant differences in the Pearson correlation for adipose and muscular tissue samples compared or the same tissue samples compared. Correlation coefficients between lipid patterns of adipose and muscular tissue samples are lower (from 0.48 to 0.86, r = 0.69 on average with 95 % confidence interval from 0.61 to 0.79). Correlation coefficients between lipid patterns in two samples of muscle or adipose tissue are higher (from 0.73 to 0.99, r = 0.93 on average with 95 % confidence interval from 0.86 to 0.97). Unpaired t-test shows differences at p-value < 0.01 Data clustering confirms the difference between muscle samples and subcutaneous fat samples. The main classes of lipids detected in the samples were triglycerides (TAG), diglycerides (DAG), phosphatidylcholines (PC), phosphatidylethanolamines (PE), phosphati-dylserines (PS), phosphatidic acids (PA), phosphatidylinositols (PI), and lysophosphatidylcholines (LPC). We have found that adipose tissue samples are enriched in triacylglycerols, while muscle tissue samples are enriched in phospholipids. To summarize, we have identified the main lipid types present in different regions of muscle and adipose tissue of pigs, and revealed the similarities and differences in the lipid composition between the two analyzed tissue types, as well as between two different types of muscles (biceps femoris and longisimus dorsi), and also between muscle and fat tissues. Considering the results obtained in this work we may conclude that liquid chromatography coupled with high-precision time-of-flight mass spectrometry efficiently produces accurate and reproducible lipidomes data. These data may be used in animal breeding, in the search for new genetic markers associated with economically important traits and in breeding programs to evaluate the traits determined by lipid composition.

    Original languageEnglish
    Pages (from-to)1262-1273
    Number of pages12
    JournalSel'skokhozyaistvennaya Biologiya
    Volume53
    Issue number6
    DOIs
    Publication statusPublished - 2018

    Keywords

    • Adipose tissue
    • Animal phenotyping
    • Duroc boars
    • High-performance liquid chromatography
    • Lipidome
    • Mass spectrometry
    • Muscle tissue
    • Pigs
    • Sus scrofa domesticus

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