Light stress photodynamics of chlorophyll-binding proteins in Arabidopsis thaliana thylakoid membranes revealed by high-resolution mass spectrometric studies

D. N. Galetskiy, J. N. Lohscheider, A. S. Kononikhin, O. N. Kharybin, I. A. Popov, I. Adamska, E. N. Nikolaev

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

In higher plants the light energy is captured by the photosynthetic pigments that are bound to photosystem I and II and their light-harvesting complex (LHC) subunits. In this study, we examined the photodynamic changes within chlorophyll-protein complexes in the thylakoid membrane of Arabidopsis thaliana leaves adapted to low light and subsequently exposed to light stress. Chlorophyll-protein complexes were isolated using sucrose density gradient centrifugation and blue-native polyacrylamid gel electrophoresis (BN-PAGE). Proteome analysis was performed using SDS-PAGE, HPLC, and high resolution mass spectrometry. We identified several rarely expressed and stress-induced chlorophyll-binding proteins, showed changes in localization of early light-induced protein family and LHC protein family members between different photosynthetic complexes and assembled/disassembled subcomplexes under light stress conditions and discuss their role in a variety of light stress-related processes.

Original languageEnglish
Pages (from-to)105-118
Number of pages14
JournalRussian Journal of Bioorganic Chemistry
Volume37
Issue number1
DOIs
Publication statusPublished - Jan 2011
Externally publishedYes

Keywords

  • Arabidopsis thaliana
  • FTICR mass spectrometry
  • light stress
  • pigment-protein complexes
  • thylakoid membranes

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