Learning from fluorescence decay dynamics: A better read out from the spectroscopic ruler

J. J. Rindermann, Y. Akhtman, J. Richardson, T. Brown, P. G. Lagoudakis

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The spectroscopic ruler is a noninvasive technique exploiting fluorescence resonance energy transfer (FRET) to determine intramolecular distances on the nm scale in proteins and other bio-molecules [1,2]. It is widely applied in the life sciences where it is used in vitro and in vivo to probe the separation between fluorescent markers attached to the molecules under investigation. Because FRET scales strongly with the separation distance and orientation between the optical dipole moments of the donor and acceptor molecules, the accuracy of the technique is limited when the molecules undergoes thermal fluctuations under physiological conditions and the orientation of the optical dipoles is not static.

Original languageEnglish
Title of host publication2011 Conference on Lasers and Electro-Optics Europe and 12th European Quantum Electronics Conference, CLEO EUROPE/EQEC 2011
DOIs
Publication statusPublished - 2011
Externally publishedYes
Event2011 Conference on Lasers and Electro-Optics Europe and 12th European Quantum Electronics Conference, CLEO EUROPE/EQEC 2011 - Munich, Germany
Duration: 22 May 201126 May 2011

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Conference

Conference2011 Conference on Lasers and Electro-Optics Europe and 12th European Quantum Electronics Conference, CLEO EUROPE/EQEC 2011
Country/TerritoryGermany
CityMunich
Period22/05/1126/05/11

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