Label-free characterization of white blood cells using fluorescence lifetime imaging and flow-cytometry: Molecular heterogeneity and erythrophagocytosis [invited]

Boris P. Yakimov, Maria A. Gogoleva, Alexey N. Semenov, Sergey A. Rodionov, Marina V. Novoselova, Alexey V. Gayer, Alexey V. Kovalev, Alexey I. Bernakevich, Victor V. Fadeev, Artashes G. Armaganov, Vladimir P. Drachev, Dmitry A. Gorin, Maxim E. Darvin, Vladislav I. Shcheslavskiy, Gleb S. Budylin, Alexander V. Priezzhev, Evgeny A. Shirshin

    Research output: Contribution to journalArticlepeer-review

    23 Citations (Scopus)

    Abstract

    Blood cell analysis is one of the standard clinical tests. Despite the widespread use of exogenous markers for blood cell quantification, label-free optical methods are still of high demand due to their possibility for in vivo application and signal specific to the biochemical state of the cell provided by native fluorophores. Here we report the results of blood cell characterization using label-free fluorescence imaging techniques and flow-cytometry. Autofluorescence parameters of different cell types – white blood cells, red blood cells, erythrophagocytic cells – are assessed and analyzed in terms of molecular heterogeneity and possibilities of differentiation between different cell types in vitro and in vivo.

    Original languageEnglish
    Article number364409
    Pages (from-to)4220-4236
    Number of pages17
    JournalBiomedical Optics Express
    Volume10
    Issue number8
    DOIs
    Publication statusPublished - 1 Aug 2019

    Fingerprint

    Dive into the research topics of 'Label-free characterization of white blood cells using fluorescence lifetime imaging and flow-cytometry: Molecular heterogeneity and erythrophagocytosis [invited]'. Together they form a unique fingerprint.

    Cite this