KillerOrange, a genetically encoded photosensitizer activated by blue and green light

Karen S. Sarkisyan, Olga A. Zlobovskaya, Dmitry A. Gorbachev, Nina G. Bozhanova, George V. Sharonov, Dmitriy B. Staroverov, Evgeny S. Egorov, Anastasia V. Ryabova, Kyril M. Solntsev, Alexander S. Mishin, Konstantin A. Lukyanov

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

Genetically encoded photosensitizers, proteins that produce reactive oxygen species when illuminated with visible light, are increasingly used as optogenetic tools. Their applications range from ablation of specific cell populations to precise optical inactivation of cellular proteins. Here, we report an orange mutant of red fluorescent protein KillerRed that becomes toxic when illuminated with blue or green light. This new protein, KillerOrange, carries a tryptophanbased chromophore that is novel for photosensitizers.We show that KillerOrange can be used simultaneously and independently from KillerRed in both bacterial and mammalian cells offering chromatic orthogonality for light-activated toxicity.

Original languageEnglish
Article numbere0145287
JournalPLoS ONE
Volume10
Issue number12
DOIs
Publication statusPublished - 1 Dec 2015
Externally publishedYes

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