Jasmonate-responsive MYB factors spatially repress rutin biosynthesis in Fagopyrum tataricum

Kaixuan Zhang, Maria D. Logacheva, Yu Meng, Jianping Hu, Dongpu Wan, Long Li, Dagmar Janovská, Zhiyong Wang, Milen I. Georgiev, Zhuo Yu, Fuyu Yang, Mingli Yan, Meiliang Zhou

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Jasmonates are plant hormones that induce the accumulation of many secondary metabolites, such as rutin in buckwheat, via regulation of jasmonate-responsive transcription factors. Here, we report on the identification of a clade of jasmonate-responsive subgroup 4 MYB transcription factors, FtMYB13, FtMYB14, FtMYB15, and FtMYB16, which directly repress rutin biosynthesis in Fagopyrum tataricum. Immunoblot analysis showed that FtMYB13, FtMYB14, and FtMYB15 could be degraded via the 26S proteasome in the COI1-dependent jasmonate signaling pathway, and that this degradation is due to the SID motif in their C-terminus. Yeast two-hybrid and bimolecular fluorescence complementation assays revealed that FtMYB13, FtMYB14, and FtMYB15 interact with the importin protein Sensitive to ABA and Drought 2 (FtSAD2) in stem and inflorescence. Furthermore, the key repressor of jasmonate signaling FtJAZ1 specifically interacts with FtMYB13. Point mutation analysis showed that the conserved Asp residue of the SID domain contributes to mediating protein-protein interaction. Protoplast transient activation assays demonstrated that FtMYB13, FtMYB14, and FtMYB15 directly repress phenylalanine ammonia lyase (FtPAL) gene expression, and FtSAD2 and FtJAZ1 significantly promote the repressing activity of FtMYBs. These findings may ultimately be promising for further engineering of plant secondary metabolism.

Original languageEnglish
Pages (from-to)1955-1966
Number of pages12
JournalJournal of Experimental Botany
Volume69
Issue number8
DOIs
Publication statusPublished - 9 Apr 2018
Externally publishedYes

Keywords

  • 26S proteasome
  • buckwheat
  • Fagopyrum
  • jasmonates
  • MYB transcription factor
  • phenylpropanoid pathway
  • tataricum

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