Investigation of the complex antibiotic INA-5812

O. A. Lapchinskaya, G. S. Katrukha, E. G. Gladkikh, V. V. Kulyaeva, P. V. Coodan, A. P. Topolyan, V. A. Alferova, V. V. Pogozheva, M. A. Sukonnikov, E. A. Rogozhin, I. A. Prokhorenko, V. A. Brylev, A. M. Korolev, M. S. Slyundina, R. S. Borisov, M. V. Serebryakova, M. V. Shuvalov, A. L. Ksenofontov, L. G. Stoyanova, I. A. OstermanA. A. Formanovsky, V. N. Tashlitsky, L. A. Baratova, A. V. Timofeeva, A. P. Tyurin

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It has been established by MALDI-TOF and ESI-MSn precision mass-spectrometry methods that the main components of the complex antibiotic are several closely related compounds, presumably of a glycopeptide nature. The fraction containing an individual component with a mass of 1845.788 Da has been characterized by UV/Vis absorbance and fluorescence spectra, amino acid analysis, and derivatization with tris(2,6-dimethoxyphenyl)methyl cation. The activity of fractions against pathogenic microbes has been studied. The results allow the supposition that the INA-5812 antibiotic complex is a glyco- or lipoglycopeptide antibiotic of a new type, which is very promising for further study.

Original languageEnglish
Pages (from-to)664-671
Number of pages8
JournalRussian Journal of Bioorganic Chemistry
Volume42
Issue number6
DOIs
Publication statusPublished - 1 Nov 2016
Externally publishedYes

Keywords

  • glycopeptide antibiotics
  • high performance liquid chromatography
  • high-resolution mass spectrometry
  • microbial metabolites

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