Intrinsic blinking of red fluorescent proteins for super-resolution microscopy

Natalia V. Klementieva, Anton I. Pavlikov, Alexander A. Moiseev, Nina G. Bozhanova, Natalie M. Mishina, Sergey A. Lukyanov, Elena V. Zagaynova, Konstantin A. Lukyanov, Alexander S. Mishin

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Single-molecule localization microscopy relies on either controllable photoswitching of fluorescent probes or their robust blinking. We have found that blinking of monomeric red fluorescent proteins TagRFP, TagRFP-T, and FusionRed occurs at moderate illumination power and matches well with camera acquisition speed. It allows for super-resolution image reconstruction of densely labelled structures in live cells using various algorithms.

Original languageEnglish
Pages (from-to)949-951
Number of pages3
JournalChemical Communications
Volume53
Issue number5
DOIs
Publication statusPublished - 2017
Externally publishedYes

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