Intra-axonal translation and retrograde trafficking of CREB promotes neuronal survival

Llewellyn J. Cox, Ulrich Hengst, Nadya G. Gurskaya, Konstantin A. Lukyanov, Samie R. Jaffrey

Research output: Contribution to journalArticlepeer-review

225 Citations (Scopus)

Abstract

During development of the nervous system, axons and growth cones contain mRNAs such as β-actin, cofilin and RhoA, which are locally translated in response to guidance cues. Intra-axonal translation of these mRNAs results in local morphological responses; however, other functions of intra-axonal mRNA translation remain unknown. Here, we show that axons of developing mammalian neurons contain mRNA encoding the cAMP-responsive element (CRE)-binding protein (CREB). CREB is translated within axons in response to nerve growth factor (NGF) and is retrogradely trafficked to the cell body. In neurons that are selectively deficient in axonal CREB transcripts, increases in nuclear pCREB, CRE-mediated transcription and neuronal survival elicited by axonal application of NGF are abolished, indicating a signalling function for axonally synthesized CREB. These studies identify a signalling role for axonally derived CREB, and indicate that signal-dependent synthesis and retrograde trafficking of transcription factors enables specific transcriptional responses to signalling events at distal axons.

Original languageEnglish
Pages (from-to)149-159
Number of pages11
JournalNature Cell Biology
Volume10
Issue number2
DOIs
Publication statusPublished - Feb 2008
Externally publishedYes

Fingerprint

Dive into the research topics of 'Intra-axonal translation and retrograde trafficking of CREB promotes neuronal survival'. Together they form a unique fingerprint.

Cite this